Which national chains operate 24-hour pharmacies — and which actually staff a pharmacist around the clock.
The other major chains — Rite Aid, Costco, Kroger, Publix — do not operate 24-hour pharmacy counters at scale. Their stores typically close between 9:00pm and 11:00pm.
CVS operates several hundred 24-hour locations clustered in major metros (Los Angeles, Chicago, Houston, Phoenix, Atlanta, Las Vegas, Miami, New York).
Walgreens still operates roughly 400 24-hour stores nationwide, the largest 24-hour pharmacy footprint of any chain.
Rite Aid does not operate true 24-hour stores at scale.
Most Walmart Supercenters returned to closing overnight after 2020 and the Walmart Pharmacy counter has never operated 24/7.
Costco does not operate any 24-hour locations.
A handful of Kroger and banner stores stay open 24 hours in the Midwest and South, but the pharmacy counter follows standard daytime hours at all locations.
Publix does not operate 24-hour stores.